Charles H. Wick, Ph.D, LTC (USA RET)
Author, Inventor, Scientist, Soldier
Identifying Microbes by
Mass Spectrometry Proteomics
Published: September 10, 2013 by CRC Press
Content: 289 Pages | 98 Illustrations
Editor: Charles H. Wick
Written for those who seek to understand how Mass Spectrometry Proteomics (MSP) can identify microbes, this book is particularly useful for understanding the success of using MSP with the ABOid software for clear unambiguous identification of bacteria, viruses and fungi. Identification is accomplished by determining the unique peptides associated with microbes and then detecting these unique peptides. This is accomplished by first using the information from all currently known sequenced microbes and then by further calculation determining their peptides and then determining those peptides unique for each microbe. It is then easy to identify microbes to strain in a complex sample. This process also allows determination of those peptides that are unique for each level in the phylogenic tree – family, genus and species. In this manner if a microbe has not been fully sequenced it is possible to determine those peptides that are unique to either family, genus or species as they are available. An unfamiliar microbe can be sorted in this manner and properly identified to the best level of identification as is available from the information determined. This is a very simple calculation and since there are many unique peptides for each microbe it is easy to identify and classify the various microbes.